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Next Generation Sequencing (NGS)

Learn what is next generation sequencing (NGS) and how it works
4.3
4.3/5
(9 reviews)
51 students
Created by

8.9

CourseMarks Score®

9.7

Freshness

8.7

Feedback

7.7

Content

Platform: Udemy
Video: 1h 5m
Language: English
Next start: On Demand

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Detailed Analysis

CourseMarks Score®

8.9 / 10

CourseMarks Score® helps students to find the best classes. We aggregate 18 factors, including freshness, student feedback and content diversity.

Freshness Score

9.7 / 10
This course was last updated on 1/2022.

Course content can become outdated quite quickly. After analysing 71,530 courses, we found that the highest rated courses are updated every year. If a course has not been updated for more than 2 years, you should carefully evaluate the course before enrolling.

Student Feedback

8.7 / 10
We analyzed factors such as the rating (4.3/5) and the ratio between the number of reviews and the number of students, which is a great signal of student commitment.

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Content Score

7.7 / 10
Video Score: 7.7 / 10
The course includes 1h 5m video content. Courses with more videos usually have a higher average rating. We have found that the sweet spot is 16 hours of video, which is long enough to teach a topic comprehensively, but not overwhelming. Courses over 16 hours of video gets the maximum score.
Detail Score: 10.0 / 10

The top online course contains a detailed description of the course, what you will learn and also a detailed description about the instructor.

Extra Content Score: 5.5 / 10

Tests, exercises, articles and other resources help students to better understand and deepen their understanding of the topic.

This course contains:

0 article.
0 resource.
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0 test.

Table of contents

Description

Over the last 56 years, researchers have been developing methods and technologies to assist in the determination of nucleic acid sequences in biological samples. The ability to sequence DNA and RNA accurately has had a great impact in numerous research fields. The sequencing of the human genome was completed in 2003, after 13 years of international collaboration and investment of USD 3 billion. The Human Genome Project used Sanger sequencing, the principal method of DNA sequencing since its invention in the 1970s. Today, the demand for sequencing is growing exponentially, with large amounts of genomic DNA needing to be analyzed quickly, cheaply, and accurately. Thanks to new sequencing technologies known collectively as Next Generation Sequencing.
Next generation sequencing (NGS), also known as high throughput sequencing or second generation sequencing or short read sequencing, is a massively parallel sequencing technology that offers ultra-high throughput, scalability, and speed. The technology is used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA.
The main difference between Sanger sequencing and NGS stems from sequencing volume, with NGS allowing the processing of millions of reactions in parallel, resulting in high-throughput, higher sensitivity, speed and reduced cost. A plethora of genome sequencing projects that took many years with Sanger sequencing methods could now be completed within hours using NGS.
There are several main steps that must be tailored to the target (RNA or DNA) and sequencing system selected. The main steps of NGS includes sample preparation (pre-processing), library preparation, PCR amplification, sequencing and data analysis (Post-processing).
Library preparation includes DNA fragmentation either enzymatically or by sonication, end repairing and adaptor ligation.
Library amplification is required so that the received signal from the sequencer is strong enough to be detected accurately. The two most common PCR amplification methods are emulsion PCR and bridge PCR.
Several competing methods of NGS have been developed by different companies including 454 Pyrosequencing, sequencing by ligation (SOLiD), ion torrent semiconductor sequencing and reversible terminator sequencing (illumina).
NGS can generate two types of reads i.e, single end reads and paired end reads, depending on method of choice.
NGS has enabled researchers to collect vast quantities of genomic sequencing data. This technology has a plethora of applications, such as: outbreak Management, diagnosing and understanding complex diseases, whole-genome sequencing, transcriptome sequencing, cancer treatments, detection of viruses, surveillance of antimicrobial resistance and many more.
Now, third (3G) and fourth (4G) generation technologies have been evolved that work on different underlying principles.
This course is a valuable resource for students and researchers related to molecular biology, forensic science, medical laboratory technology, biotechnology, and genetics.
Start your learning journey now and explore the hidden truth about sequencing technology!

You will learn

✓ Introduction and Historical Background of Next Generation Sequencing
✓ What is Next Generation Sequencing (NGS)?
✓ Key Principle behind NGS
✓ Difference between Sanger sequencing and NGS
✓ Sample preparation (Pre-processing) for NGS
✓ Library Preparation for NGS
✓ PCR Amplification for NGS including Emulsion PCR and Bridge PCR
✓ 454 Pyrosequencing approcah
✓ Sequencing by ligation (SOLiD) approach
✓ Ion torrent semiconductor sequencing approach
✓ Reversible terminator sequencing (illumina) approach
✓ Sequencing read types including single end and paired end reads
✓ Data Analysis (Post-processing) for NGS
✓ key considerations when selecting sequencing strategy
✓ Strengths and Limitations of Next Generation Sequencing
✓ Applications of Next Generation Sequencing
✓ Third and Fourth Generation Technologies

Requirements

• Motivation to learn
• Basic knowledge of biology and molecular biology
• Interested in learning latest technologies

This course is for

• Students who want to go for bioltechnologist, lab technologist and molecular biologists job.
• The course is designed for researchers of molecular biology, biotechnology, and related disciplines.
• Students who wants to persude their career in sequencing analysis, forensic science, research, molecular biology, clinincal analysis and genetics.

How much does the Next Generation Sequencing (NGS) course cost? Is it worth it?

The course costs $14.99. And currently there is a 40% discount on the original price of the course, which was $24.99. So you save $10 if you enroll the course now.

Does the Next Generation Sequencing (NGS) course have a money back guarantee or refund policy?

YES, Next Generation Sequencing (NGS) has a 30-day money back guarantee. The 30-day refund policy is designed to allow students to study without risk.

Are there any SCHOLARSHIPS for this course?

Currently we could not find a scholarship for the Next Generation Sequencing (NGS) course, but there is a $10 discount from the original price ($24.99). So the current price is just $14.99.

Who is the instructor? Is Anum Ahmad, PhD a SCAM or a TRUSTED instructor?

Anum Ahmad, PhD has created 8 courses that got 51 reviews which are generally positive. Anum Ahmad, PhD has taught 854 students and received a 4.3 average review out of 51 reviews. Depending on the information available, Anum Ahmad, PhD is a TRUSTED instructor.
Educator
I am a PhD (microbiology) from a reputed university. I have been teaching biology and chemistry for more than 7 years in prestigious institutes.
Biology and chemistry are the fields that need a lot of toil and laser-sharp focused attention. These subjects could be difficult as mountain climbing and easy as watching your favorite TV serial depending on who your teacher is.
I aim to make these subjects as easy and interesting as possible. Linking these subjects to daily life is an interesting way to retain the knowledge of these subjects. Keeping these things in mind, I have designed these courses to aid the students.
I am not only a teacher but also a researcher. I worked on different aspects of life sciences including:
Biotechnology
Rumen microbiota
Animal nutrition
Molecular biology (cloning and expression of genes)
My research experience has opened a vast horizon of knowledge in front of me, which I am willing to share with my students to contribute towards their self-achievement and value addition.

8.9

CourseMarks Score®

9.7

Freshness

8.7

Feedback

7.7

Content

Platform: Udemy
Video: 1h 5m
Language: English
Next start: On Demand

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