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Blotting Techniques made easy

Detailed explination
5.0
5.0/5
(1 reviews)
2 students
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9.4

CourseMarks Score®

9.9

Freshness

10.0

Feedback

7.8

Content

Platform: Udemy
Video: 2h 43m
Language: English
Next start: On Demand

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Detailed Analysis

CourseMarks Score®

9.4 / 10

CourseMarks Score® helps students to find the best classes. We aggregate 18 factors, including freshness, student feedback and content diversity.

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9.9 / 10
This course was last updated on 11/2021.

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10.0 / 10
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Content Score

7.8 / 10
Video Score: 8.0 / 10
The course includes 2h 43m video content. Courses with more videos usually have a higher average rating. We have found that the sweet spot is 16 hours of video, which is long enough to teach a topic comprehensively, but not overwhelming. Courses over 16 hours of video gets the maximum score.
Detail Score: 10.0 / 10

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Extra Content Score: 5.5 / 10

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Table of contents

Description

Part 1:
Definition and introduction
3 types of Blotting techniques
   Southern
   Northern
   Western
Sothern: 6 steps

    DNA Digestion
    Gel Electrophoresis
   Blotting
   Probelabelling
   Hybridization
   Detection
Sothern blotting Diagram
Part 2:
Sothern Blotting explanation
Part 3:

Sothern Blotting 10 steps
Factors affecting
Stringent conditions
Membrane for bloat transfer
Applications
Part 4:
Applications
strengths and limitations
Part 5:
Northern Blotting
steps: 8
RNA isolation
probe generation
Agarosegelelectrophoresis
immobilization
Hybridization
washing
detection
re-probing
diagram
Part 6:
Norther blotting applications
advantages and disadvantages
Part 7:
Western blotting
Dentition
protocol
steps
Western blotting buffer preparation:
sample preparations
Gelelectrophoresis
protein transfer
Membrane blocking
antibody incubation
western blotting detection
Data analysis
W.B diagram

Part 8:
procedure
explanation
Part 9:
what is SNOW Drop

Detecting Phosphorylation States of Proteins
Many proteins are post-translationally modified by kinases, which add phosphate groups to their substrates. Western blotting is great for detecting the presence of phosphorylated proteins. Phosphorylated proteins become heavier, due to the added weight of the phosphate group, so they often migrate more slowly than their un-phosphorylated forms.
Detecting Changes in Protein Levels Across Treatment Groups
Detecting the presence and absence of a protein may seem like a simplistic assay, but when each sample represents a different treatment group, western blotting can be very informative about the amount of each protein in treatment. This also applies to detecting how a certain treatment changes the post-translational modification of a protein (i.e. phosphorylation, ubiquitination, etc.).
Detecting Changes in Protein Levels Across Time Points
Western blotting is incredibly informative for determining the effect of time on a protein. For example, if each sample is a protein mixture of cells that are in different phases of the cell cycle, then western blotting will reveal how much a protein is present or absent during each phase.
Detecting Truncated Isoforms of Proteins
Many proteins are cleaved in order to be activated, or have naturally occurring truncation isoforms. Each isoform may have a different level of activity, a different target protein, or represent a different cellular state. Western blotting is great for detecting the ratio of truncated to normal isoforms of a protein.
Detecting Tagged Proteins
Some proteins are engineered, through the process of molecular cloning, to contain short sequences of amino acids that serve as a tag. Common tags include the HA-tag and the Myc-tag. These tags serve as a foreign protein epitope that does not naturally occur in the biological system being studied. Thus, the tag makes the protein easy to detect compared to all other naturally occurring proteins. An antibody directed to the tag will identify the presence and amount of the tagged protein in the western blot.
and many more in slide explanation

You will learn

✓ Part 1:Definition and introduction
✓ 3 types of Blotting techniques
✓ Southern
✓ Northern
✓ Western
✓ Sothern: 6 steps
✓ Part 2:Sothern Blotting explanation
✓ Part 3:Sothern Blotting 10 steps
✓ Part 4:Applications
✓ strengths and limitations
✓ Part 5: Northern Blotting
✓ steps: 8
✓ Part 6:Norther blotting applications
✓ advantages and disadvantages
✓ Part 7: Western blotting
✓ Dentition
✓ steps
✓ protocol
✓ Data analysis
✓ Part 8:procedure
✓ explanation
✓ Part 9:
✓ what is SNOW Drop

Requirements

• No requirements
• Any Biology student can learn

This course is for

• Biology
• Micro Biology
• Bio-technology
• Bio-chemistry
• Pharmacy
• Medicine

How much does the Blotting Techniques made easy course cost? Is it worth it?

The course costs $12.99. And currently there is a 85% discount on the original price of the course, which was $84.99. So you save $72 if you enroll the course now.

Does the Blotting Techniques made easy course have a money back guarantee or refund policy?

YES, Blotting Techniques made easy has a 30-day money back guarantee. The 30-day refund policy is designed to allow students to study without risk.

Are there any SCHOLARSHIPS for this course?

Currently we could not find a scholarship for the Blotting Techniques made easy course, but there is a $72 discount from the original price ($84.99). So the current price is just $12.99.

Who is the instructor? Is Sowjanya Chollangi a SCAM or a TRUSTED instructor?

Sowjanya Chollangi has created 8 courses that got 6 reviews which are generally positive. Sowjanya Chollangi has taught 18 students and received a 3.6 average review out of 6 reviews. Depending on the information available, Sowjanya Chollangi is a TRUSTED instructor.
Professor in Life science, MSc – Biotechnology, MEd
I have total 7+year of teaching experience in life sciences. I have educates students from different life science backgrounds like Botany, Zoology, Microbiology, Bio-chemistry and Biotechnology. I have extensive teaching experience in Bio-technology and related concepts. I worked as professors on Bio-technology, Bio-Chemistry and Botany.can able to explain in practical manner and good demonstrative skills.  any life science student can ask queries.Show moreShow less

9.4

CourseMarks Score®

9.9

Freshness

10.0

Feedback

7.8

Content

Platform: Udemy
Video: 2h 43m
Language: English
Next start: On Demand

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